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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up noted but in depth nucleotide sequence details will not be still offered.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best results are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody versus the antibody on your own, you can see which staining is particular.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each mitotic celebration. Cyclin E types a complex with and functions as a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates for the G1-S section boundary and is degraded as cells development by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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